Accidental selection and intentional restoration of sporulation - deficient 2 mutants of Bacillus anthracis
نویسندگان
چکیده
19 We demonstrate the frequent accidental enrichment of spontaneous sporulation-deficient mutants 20 of Bacillus anthracis on solid medium and identify contributing factors. Mutations in spo0A, 21 encoding the master regulator of sporulation initiation, were found in 38 of 53 mutants. 22 Transductions using bacteriophage CP51 propagated on sporogenic bacteria allowed restoration 23 of sporulation phenotypes. 24 on N ovem er 2, 2017 by gest ht://aem .sm .rg/ D ow nladed fom
منابع مشابه
Accidental selection and intentional restoration of sporulation-deficient Bacillus anthracis mutants.
We demonstrate the frequent accidental enrichment of spontaneous sporulation-deficient mutants of Bacillus anthracis on solid medium and identify contributing factors. Mutations in spo0A, encoding the master regulator of sporulation initiation, were found in 38 of 53 mutants. Transductions using bacteriophage CP51 propagated on sporogenic bacteria allowed for the restoration of sporulation phen...
متن کاملCharacterization of sporulation histidine kinases of Bacillus anthracis.
The initiation of sporulation in Bacillus species is regulated by the phosphorelay signal transduction pathway, which is activated by several histidine sensor kinases in response to cellular and metabolic signals. Comparison of the protein components of the phosphorelay between Bacillus subtilis and Bacillus anthracis revealed high homology in the phosphorelay orthologs of Spo0F, Spo0B, and Spo...
متن کاملReplication Initiation Proteins Regulate a Developmental Checkpoint in Bacillus subtilis
We identified a signaling pathway that prevents initiation of sporulation in Bacillus subtilis when replication initiation is impaired. We isolated mutations that allow a replication initiation mutant (dnaA) to sporulate. These mutations affect a small open reading frame, sda, that was overexpressed in replication initiation mutants and appears to be directly regulated by DnaA. Mutations in rep...
متن کاملCharacterization of Bacillus anthracis germinant receptors in vitro.
Bacillus anthracis begins its infectious cycle as a metabolically dormant cell type, the endospore. Upon entry into a host, endospores rapidly differentiate into vegetative bacilli through the process of germination, thus initiating anthrax. Elucidation of the signals that trigger germination and the receptors that recognize them is critical to understanding the pathogenesis of B. anthracis. In...
متن کاملAn overlap between the control of programmed cell death in Bacillus anthracis and sporulation.
The Staphylococcus aureus cid and lrg operons have been shown to control cell death and lysis in a manner thought to be analogous to programmed cell death (apoptosis) in eukaryotic organisms. Although orthologous operons are present in a wide variety of bacterial species, members of the Bacillus cereus group are unique in that they have a total of four cid-/lrg-like operons. Two of these operon...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010